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  • Featured Product
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GLUD2 Polyclonal antibody

GLUD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14462-1-AP

Synonyms

GLUDP1, GLUD 2, GDH2, GDH 2, EC:1.4.1.3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GLUD2 Polyclonal antibody (14462-1-AP)

    WB analysis of HepG2 using 14462-1-AP

    WB result of GLUD2 antibody (14462-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUD2 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using GLUD2 Polyclonal antibody (14462-1-AP)

    WB analysis using 14462-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GLUD2 Polyclonal antibody (14462-1-AP)

    IHC staining of human prostate cancer using 14462-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GLUD2 Polyclonal antibody (14462-1-AP)

    IHC staining of human prostate cancer using 14462-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GLUD2 Polyclonal antibody (14462-1-AP)

    IF Staining of HepG2 using 14462-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GLUD2 antibody (14462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse eye tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14462-1-AP targets GLUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUD2 fusion protein Ag5748
Full Name glutamate dehydrogenase 2
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC050732
Gene Symbol GLUD2
Gene ID (NCBI) 2747
RRIDAB_2110642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLUD2 antibody 14462-1-APDownload protocol
IHC protocol for GLUD2 antibody 14462-1-APDownload protocol
IF protocol for GLUD2 antibody 14462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Metab

Gut microbiome-derived ammonia modulates stress vulnerability in the host

Authors - Pei Wang
View Article
humanIHC

Transl Oncol

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.

Authors - Xuyong Wei
View Article
humanWB

Amino Acids

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Authors - Chunhong Cui
View Article