• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using GLS2 Polyclonal antibody (20171-1-AP)

    WB analysis using 20171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GLS2 Polyclonal antibody (20171-1-AP)

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GLS2 Polyclonal antibody (20171-1-AP)

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLS2 Polyclonal antibody

GLS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20171-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20171-1-AP targets GLS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLS2 fusion protein Ag14003 Predict reactive species
Full Name glutaminase 2 (liver, mitochondrial)
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight66-70 kDa
GenBank Accession NumberBC059370
Gene Symbol GLS2
Gene ID (NCBI) 27165
RRIDAB_3085600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.

Protocols

Product Specific Protocols
WB protocol for GLS2 antibody 20171-1-APDownload protocol
IHC protocol for GLS2 antibody 20171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols