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  • Western Blot (WB) analysis of various lysates using GGTLC1 Polyclonal antibody (17537-1-AP)

    WB analysis using 17537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17537-1-AP (GGTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GGTLC1 Polyclonal antibody (17537-1-AP)

    IF Staining of HepG2 using 17537-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GGTLC1 antibody (17537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GGTLC1 Polyclonal antibody

GGTLC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17537-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GGTL6, GGTLA3, GGTLA4, GGTLC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHepG2 cells, L02 cells, human urine
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17537-1-AP targets GGTLC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGTLC1 fusion protein Ag11592 Predict reactive species
Full Name gamma-glutamyltransferase light chain 1
Calculated Molecular Weight 225 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC040904
Gene Symbol GGTLC1
Gene ID (NCBI) 92086
RRIDAB_3669277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX51
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma-glutamyltransferase light chain 1 (GGTLC1), initially named GGTL6, then GGTLA4, and later GGTLC1 in 2008, is a member of the gamma-glutamyl transferase (GGT) family. The most ubiquitously expressed human GGT gene, GGT1, encodes a single transmembrane polypeptide that is post-translationally processed to form a heavy and a light chain. Knockdown of GGTLC1 in Endometrial cancer (EC) cells was shown to induce G0/G1 phase arrest and apoptosis, underscoring the significance of GGTLC1 as a key regulator of these cellular processes in EC (PMID: 38841516).

Protocols

Product Specific Protocols
WB protocol for GGTLC1 antibody 17537-1-APDownload protocol
IF protocol for GGTLC1 antibody 17537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols