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  • KD/KO Validated

GGCX Polyclonal antibody

GGCX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 16209-1-AP

Synonyms

gamma glutamyl carboxylase, GC, GGCX, VKCFD1



Tested Applications

Positive WB detected inMCF7 cells, HEK-293 cells, HepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16209-1-AP targets GGCX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGCX fusion protein Ag9185
Full Name gamma-glutamyl carboxylase
Calculated Molecular Weight 758 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC013979
Gene Symbol GGCX
Gene ID (NCBI) 2677
RRIDAB_2110874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

Protocols

Product Specific Protocols
WB protocol for GGCX antibody 16209-1-APDownload protocol
IHC protocol for GGCX antibody 16209-1-APDownload protocol
IP protocol for GGCX antibody 16209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

GGCX and VKORC1 inhibit osteocalcin endocrine functions.

Authors - Mathieu Ferron

Blood

Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders.

Authors - Jian-Ke Tie
humanWB

Methods Enzymol

Functional Study of the Vitamin K Cycle Enzymes in Live Cells.

Authors - J-K Tie
  • KO Validated
humanWB

JCI Insight

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.

Authors - Julie Lacombe
humanWB

Oncotarget

Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells.

Authors - Sarah Beaudin
human,mouseWB,IP

Cell Rep

Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells

Authors - Julie Lacombe