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  • Western Blot (WB) analysis of mouse heart tissue using GFRA3 Polyclonal antibody (25379-1-AP)

    WB analysis of mouse heart using 25379-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25379-1-AP (GFRA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using GFRA3 Polyclonal antibody (25379-1-AP)

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GFRA3 Polyclonal antibody (25379-1-AP)

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 40x lens).

GFRA3 Polyclonal antibody

GFRA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25379-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GDNF family receptor alpha 3, GDNF receptor alpha 3, GDNFR alpha 3, GFR alpha 3, GFRA3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25379-1-AP targets GFRA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFRA3 fusion protein Ag13253 Predict reactive species
Full Name GDNF family receptor alpha 3
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC037951
Gene Symbol GFRA3
Gene ID (NCBI) 2676
RRIDAB_2880050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA3 is a receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). It mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. GFRA3 has two isoforms with MW 45 kDa and 41 kDa. It can be cleaved to be a mature form with MW 41 kDa or 37 kDa.

Protocols

Product Specific Protocols
WB protocol for GFRA3 antibody 25379-1-APDownload protocol
IHC protocol for GFRA3 antibody 25379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols