Filter: AllIPIHC

IP experiment of mouse skeletal muscle using 16494-1-AP
IP result of anti-GDPD5 (IP:16494-1-AP, 4ug; Detection:16494-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.
IHC staining of human spleen using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 16494-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

GDPD5 Polyclonal antibody

Name: GDPD5 Polyclonal antibody
Brand: Proteintech
SKU: 16494-1-AP

GDPD5 Polyclonal Antibody for IP, IHC, ELISA

Cat No. 16494-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA

Synonyms

GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human spleen tissue, human kidney tissue, human ovary tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16494-1-AP targets GDPD5 in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GDPD5 fusion protein Ag9622 Predict reactive species
Full Name	glycerophosphodiester phosphodiesterase domain containing 5
Calculated Molecular Weight	127 aa, 13 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC020203
Gene Symbol	GDPD5
Gene ID (NCBI)	81544
RRID	AB_2111683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WTR4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.