Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15629-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 15629-1-AP
IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.
IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.
IHC staining of human liver cancer using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse liver tissue, rat liver tissue, pig liver tissue
Positive IP detected in
mouse liver tissue
Positive IHC detected in
human liver cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
This antibody is not recommended for WB detection of human samples.
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human.
Improved lipogenesis gene expression in liver is associated with elevated plasma angiotensin 1-7 after AT1 receptor blockade in insulin-resistant OLETF rats
Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15629-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 15629-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse liver using 15629-1-AP
IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
KD/KO VALIDATED
GCK Polyclonal antibody
Catalog Number
15629-1-AP
Citations
3
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500
Applications
WB, IHC, IP, ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
