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  • KD/KO Validated

GBF1 Polyclonal antibody

GBF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25183-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25183-1-AP targets GBF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBF1 fusion protein Ag16579 Predict reactive species
Full Name golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
Calculated Molecular Weight 1859 aa, 206 kDa
Observed Molecular Weight 206 kDa
GenBank Accession NumberBC117681
Gene Symbol GBF1
Gene ID (NCBI) 8729
RRIDAB_2879948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GBF1 antibody 25183-1-APDownload protocol
IHC protocol for GBF1 antibody 25183-1-APDownload protocol
IF protocol for GBF1 antibody 25183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

TBC1D23 mediates Golgi-specific LKB1 signaling

Authors - Yingfeng Tu
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
humanWB

Sci Rep

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Authors - Xiaobo Lei
  • KD Validated
humanIF

J Virol

Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1

Authors - Junki Hirano
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