Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
19958-1-AP targets GAC-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutaminase |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | AF158555 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_10640899 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IHC protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IP protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| WB protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection. | ||
Cell Stem Cell Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Blood Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. | ||
Elife Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor | ||
Cell Death Discov O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Daniel (Verified Customer) (09-16-2019) | The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.
 |
