• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10060-1-AP

Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 10060-1-AP

    WB analysis of MCF-7 using 10060-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10060-1-AP

    WB analysis of HEK-293 using 10060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 10060-1-AP

    WB analysis of human brain using 10060-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 10060-1-AP

    WB analysis of MCF-7 using 10060-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 10060-1-AP

    WB analysis of MDA-MB-453s using 10060-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of T-47D using 10060-1-AP

    WB analysis of T-47D using 10060-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 10060-1-AP

    WB analysis of Jurkat using 10060-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 10060-1-AP

    WB analysis of human testis using 10060-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10060-1-AP

    WB analysis of HeLa using 10060-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10060-1-AP

    WB analysis of HEK-293 using 10060-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10060-1-AP

    IP experiment of MCF-7 using 10060-1-AP

    IP Result of anti-FKBPL (IP:10060-1-AP, 3ug; Detection:10060-1-AP 1:800) with MCF-7 cells lysate 2500ug.

  • IHC staining of human testis using 10060-1-AP

    IHC staining of human testis using 10060-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 10060-1-AP

    IHC staining of human testis using 10060-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10060-1-AP

    IHC staining of human breast cancer using 10060-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10060-1-AP

    IHC staining of human breast cancer using 10060-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells, HEK-293 cells, human testis tissue, HeLa cells, MDA-MB-453s cells, human brain tissue, Jurkat cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10060-1-AP targets FKBPL in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBPL fusion protein Ag0112
Full Name FK506 binding protein like
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 42-48 kDa, 38-40 kDa
GenBank accession numberBC004168
Gene symbol FKBPL
Gene ID (NCBI) 63943
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 Kda and 48 Kda by western blot.

Protocols

Product Specific Protocols
WB protocol for FKBPL antibody 10060-1-APDownload protocol
IHC protocol for FKBPL antibody 10060-1-APDownload protocol
IP protocol for FKBPL antibody 10060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

FKBPL and its peptide derivatives inhibit endocrine therapy resistant cancer stem cells and breast cancer metastasis by downregulating DLL4 and Notch4.

Authors - Lana McClements
View Article
human,mouseWB,IHC

Reprod Biol Endocrinol

Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study.

Authors - Sunnotel Olaf O
View Article
rat, humanWB,IF

Biol Sex Differ

Characterisation of cardiac health in the reduced uterine perfusion pressure model and a 3D cardiac spheroid model, of preeclampsia.

Authors - Claire Richards
View Article
humanWB,IF

Front Endocrinol (Lausanne)

FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial Function.

Authors - Abdelrahim Alqudah
View Article
humanWB,IF

PLoS One

The anti-migratory effects of FKBPL and its peptide derivative, AD-01: regulation of CD44 and the cytoskeletal pathway.

Authors - Yakkundi Anita A
  • KD Validated
View Article
humanWB

J Biol Chem

PMID: 20018861

Authors - Bublik Débora Rosa DR
View Article