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FKBPL Polyclonal antibody
FKBPL Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ChIP,ELISA
Conjugate
Unconjugated
Cat no : 10060-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, T-47D cells, HEK-293 cells, human testis tissue, HeLa cells, MDA-MB-453s cells, human brain tissue, Jurkat cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 15 publications below |
IHC | See 4 publications below |
IF | See 6 publications below |
ChIP | See 1 publications below |
Product Information
10060-1-AP targets FKBPL in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FKBPL fusion protein Ag0112 |
Full Name | FK506 binding protein like |
Calculated molecular weight | 349 aa, 38 kDa |
Observed molecular weight | 42-48 kDa, 38-40 kDa |
GenBank accession number | BC004168 |
Gene symbol | FKBPL |
Gene ID (NCBI) | 63943 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 Kda and 48 Kda by western blot.
Protocols
Product Specific Protocols | |
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WB protocol for FKBPL antibody 10060-1-AP | Download protocol |
IHC protocol for FKBPL antibody 10060-1-AP | Download protocol |
IP protocol for FKBPL antibody 10060-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
BMC Cancer FKBPL and its peptide derivatives inhibit endocrine therapy resistant cancer stem cells and breast cancer metastasis by downregulating DLL4 and Notch4. | ||
Reprod Biol Endocrinol Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study. | ||
Biol Sex Differ Characterisation of cardiac health in the reduced uterine perfusion pressure model and a 3D cardiac spheroid model, of preeclampsia. | ||
Front Endocrinol (Lausanne) FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial Function. | ||
PLoS One The anti-migratory effects of FKBPL and its peptide derivative, AD-01: regulation of CD44 and the cytoskeletal pathway.
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