- Featured Product
- KD/KO Validated
FKBP5 Polyclonal antibody
FKBP5 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14155-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HEK-293 cells, mouse thymus tissue |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 11 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Product Information
14155-1-AP targets FKBP5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FKBP5 fusion protein Ag5337 |
Full Name | FK506 binding protein 5 |
Calculated Molecular Weight | 457 aa, 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC042605 |
Gene Symbol | FKBP5 |
Gene ID (NCBI) | 2289 |
RRID | AB_2231625 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FKBP5 antibody 14155-1-AP | Download protocol |
IHC protocol for FKBP5 antibody 14155-1-AP | Download protocol |
IP protocol for FKBP5 antibody 14155-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell Decoding the temporal and regional specification of microglia in the developing human brain. | ||
EMBO J USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.
| ||
Nutrients Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus | ||
RNA The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly. | ||
Biochem Pharmacol Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice. |