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  • Featured Product
  • KD/KO Validated

FBL Polyclonal antibody

FBL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 16021-1-AP

Synonyms

RNU3IP1, FLRN, fibrillarin, FIB1, FIB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBL Polyclonal antibody (16021-1-AP)

    WB analysis using 16021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using FBL Polyclonal antibody (16021-1-AP)

    WB analysis of mouse kidney using 16021-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FBL Polyclonal antibody (16021-1-AP)

    WB analysis of mouse liver using 16021-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using FBL Polyclonal antibody (16021-1-AP)

    WB analysis of A375 using 16021-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using FBL Polyclonal antibody (16021-1-AP)

    IP experiment of A375 using 16021-1-AP

    IP result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBL Polyclonal antibody (16021-1-AP)

    IHC staining of mouse brain using 16021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBL Polyclonal antibody (16021-1-AP)

    IHC staining of mouse brain using 16021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBL Polyclonal antibody (16021-1-AP)

    IF Staining of HepG2 using 16021-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBL Polyclonal antibody (16021-1-AP)

    IF Staining of HepG2 using 16021-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBL Polyclonal antibody (16021-1-AP)

    IF Staining of HepG2 using 16021-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA375 cells, mouse liver tissue, mosue kidney tissue
Positive IP detected inA375 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16021-1-AP targets FBL in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBL fusion protein Ag8841
Full Name fibrillarin
Calculated Molecular Weight 321 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
RRIDAB_2105788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FBL antibody 16021-1-APDownload protocol
IHC protocol for FBL antibody 16021-1-APDownload protocol
IF protocol for FBL antibody 16021-1-APDownload protocol
IP protocol for FBL antibody 16021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
View Article
humanWB

Mol Cell

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

Authors - Xin Wang
  • KD Validated
View Article
humanWB

Breast Cancer Res

U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression

Authors - Wenrong Zhang
View Article
humanWB,IF

Cell Biol Toxicol

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells

Authors - Jingyi Cui
View Article
IF

Commun Biol

Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Authors - Tetsuya Yamamoto
View Article
humanWB

Oncotarget

Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.

Authors - Xing Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tatyana (Verified Customer) (03-11-2023)

Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, U2OS cells
FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart
FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mIMCD3
FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)
FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human satelllite muscle cells
FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)