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  • Featured Product
  • KD/KO Validated

Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 26056-1-AP

Synonyms

Villin-2, VIL2, EZR

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Ezrin Polyclonal antibody (26056-1-AP)

    WB analysis of HeLa using 26056-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Ezrin Polyclonal antibody (26056-1-AP)

    WB analysis using 26056-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Ezrin Polyclonal antibody (26056-1-AP)

    WB analysis of mouse kidney using 26056-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Ezrin Polyclonal antibody (26056-1-AP)

    WB analysis of NIH/3T3 using 26056-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Ezrin Polyclonal antibody (26056-1-AP)

    IP experiment of HeLa using 26056-1-AP

    IP result of anti-Ezrin (IP:26056-1-AP, 4ug; Detection:26056-1-AP 1:2000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using Ezrin Polyclonal antibody (26056-1-AP)

    IHC staining of mouse stomach using 26056-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using Ezrin Polyclonal antibody (26056-1-AP)

    IHC staining of mouse stomach using 26056-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using Ezrin Polyclonal antibody (26056-1-AP)

    IHC staining of mouse stomach using 26056-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:8000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Ezrin Polyclonal antibody (26056-1-AP)

    IHC staining of human small intestine using 26056-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Ezrin Polyclonal antibody (26056-1-AP)

    IHC staining of human small intestine using 26056-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ezrin Polyclonal antibody (26056-1-AP)

    IF Staining of HeLa using 26056-1-AP

    Immunofluorescent analysis of HeLa cells using 26056-1-AP (Ezrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Ezrin Polyclonal antibody (26056-1-AP)

    FC experiment of HeLa using 26056-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Ezrin Polyclonal antibody (26056-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26056-1-AP targets Ezrin in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ezrin fusion protein Ag23530
Full Name ezrin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC013903
Gene Symbol Ezrin
Gene ID (NCBI) 7430
RRIDAB_2722561
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Ezrin antibody 26056-1-APDownload protocol
IHC protocol for Ezrin antibody 26056-1-APDownload protocol
IF protocol for Ezrin antibody 26056-1-APDownload protocol
IP protocol for Ezrin antibody 26056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Acta Neuropathol

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Authors - Matthew J Broadhead
View Article
humanWB

Proc Natl Acad Sci U S A

Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.

Authors - Krishnendu Khan
  • KO Validated
View Article
mouseWB

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
View Article
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan
  • KD Validated
View Article
mouseWB

J Biol Chem

Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.

Authors - Songhua Li
View Article
pigWB,IF

J Virol

Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin

Authors - Shujuan Zhang
View Article