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Entactin Monoclonal antibody

Entactin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11G1

Cat no : 67310-1-Ig

Synonyms

Entactin, NID, NID 1, NID1, nidogen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of mouse kidney using 67310-1-Ig

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of mouse heart using 67310-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of rat kidney using 67310-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of HeLa using 67310-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of human placenta using 67310-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of pig heart using 67310-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Entactin Monoclonal antibody (67310-1-Ig)

    WB analysis of rat heart using 67310-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Entactin Monoclonal antibody (67310-1-Ig)

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Entactin Monoclonal antibody (67310-1-Ig)

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67310-1-Ig targets Entactin in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Entactin fusion protein Ag4739
Full Name nidogen 1
Calculated Molecular Weight 1247 aa, 136 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC045606
Gene Symbol Entactin/NID
Gene ID (NCBI) 4811
RRIDAB_2882570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Entactin antibody 67310-1-IgDownload protocol
IHC protocol for Entactin antibody 67310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols