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- KD/KO Validated
MIG6; ERRFI1 Polyclonal antibody
MIG6; ERRFI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11630-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
11630-1-AP targets MIG6; ERRFI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIG6; ERRFI1 fusion protein Ag2202 |
| Full Name | ERBB receptor feedback inhibitor 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC025337 |
| Gene Symbol | MIG6 |
| Gene ID (NCBI) | 54206 |
| RRID | AB_2101525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| IHC protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| IF protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors. | ||
Proc Natl Acad Sci U S A Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation. | ||
Nucleic Acids Res A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells. | ||
Aging Cell METTL14 affects UVB-induced human dermal fibroblasts photoaging via miR-100-3p biogenesis in an m6 A-dependent manner | ||
EMBO Rep MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer. | ||
Arch Pharm Res Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC
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