Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24344-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 24344-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 24344-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24344-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 24344-1-AP
IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of human liver cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.
IF Staining of human liver cancer using 24344-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human liver cancer using 24344-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 24344-1-AP
Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue, human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human liver cancer tissue
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).
Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization
Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24344-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 24344-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 24344-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24344-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.
IHC staining of human pancreas using 24344-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.
IP Figures
IP experiment of mouse liver using 24344-1-AP
IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IF-P Figures
IF Staining of human liver cancer using 24344-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human liver cancer using 24344-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of HeLa using 24344-1-AP
Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ERP29 Polyclonal antibody
Catalog Number
24344-1-AP
Citations
2
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
