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WB analysis of HepG2 using 16108-1-AP
WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16108-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16108-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16108-1-AP
IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16108-1-AP
Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ERGIC1 Polyclonal antibody

Name: ERGIC1 Polyclonal antibody
Brand: Proteintech
SKU: 16108-1-AP

ERGIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16108-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ERGIC 32, ERGIC1, ERGIC32, KIAA1181, NET24

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, L02 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

16108-1-AP targets ERGIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERGIC1 fusion protein Ag9025 Predict reactive species
Full Name	endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1
Calculated Molecular Weight	290 aa, 33 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC012766
Gene Symbol	ERGIC1
Gene ID (NCBI)	57222
RRID	AB_2098435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969X5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.

Protocols

Product Specific Protocols
WB protocol for ERGIC1 antibody 16108-1-AP	Download protocol
IHC protocol for ERGIC1 antibody 16108-1-AP	Download protocol
IF protocol for ERGIC1 antibody 16108-1-AP	Download protocol
IP protocol for ERGIC1 antibody 16108-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Rep Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522. Authors - Shen Mynn Tan View Article
human	WB	Cell Rep Proteomic Profiling of Mammalian COPII and COPI Vesicles. Authors - Frank Adolf View Article
human	WB,IF	Nat Commun Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2 Authors - Karsten Nalbach View Article
human	WB,IF	Cell Rep E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC Authors - Feiyu Tang KD Validated View Article