WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16108-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16108-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16108-1-AP
IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.
IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16108-1-AP
Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.
WB result of ERGIC1 antibody (16108-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC1 transfected HepG2 cells.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16108-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16108-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16108-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16108-1-AP (ERGIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16108-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16108-1-AP (ERGIC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 16108-1-AP
IP result of anti-ERGIC1 (IP:16108-1-AP, 3ug; Detection:16108-1-AP 1:500) with HepG2 cells lysate 1720ug.
IF/ICC Figures
IF Staining of HepG2 using 16108-1-AP
Immunofluorescent analysis of HepG2 cells using 16108-1-AP (ERGIC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
ERGIC1 Polyclonal antibody
Catalog Number
16108-1-AP
Citations
4
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet