Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 55215-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
55215-1-AP targets EPHA5 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 55215-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of SH-SY5Y using 55215-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
EPHA5 Polyclonal antibody
Catalog Number
55215-1-AP
Citations
-
Dilutions
IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
IHC, IF/ICC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
