EDEM1 Polyclonal antibody

EDEM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 26226-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

EDEM, EDEM1, KIAA0212

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

26226-1-AP targets EDEM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDEM1 fusion protein Ag24046 Predict reactive species
Full Name ER degradation enhancer, mannosidase alpha-like 1
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC019088
Gene Symbol EDEM1
Gene ID (NCBI) 9695
RRIDAB_2880433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EDEM1 antibody 26226-1-APDownload protocol
IHC protocol for EDEM1 antibody 26226-1-APDownload protocol
IP protocol for EDEM1 antibody 26226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.

Authors - Chengdong Liu
humanWB

Front Mol Biosci

Glutamate-Cysteine Ligase Catalytic Subunit Attenuated Hepatitis C Virus-Related Liver Fibrosis and Suppressed Endoplasmic Reticulum Stress.

Authors - Na Fu
humanWB

J Cell Biochem

Erdj3 has an essential role for Z variant Alpha-1-Antitrypsin Degradation.

Authors - Nazli Khodayari
mouseWB

bioRxiv

A new role for IFRD1 in regulation of ER stress in bladder epithelial homeostasis

Authors - Bisiayo E Fashemi
mouseWB

iScience

IFRD1 is required for maintenance of bladder epithelial homeostasis

Authors - Bisiayo E Fashemi
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