Filter: AllWBIPIHCIF-PIF-FroIF/ICC

WB analysis of A431 using 20874-1-AP
WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.
WB analysis using 20874-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.
WB analysis using 20874-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 20874-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 20874-1-AP
MCF-7 (E-cadherin+), A431 (E-cadherin+), HeLa (E-cadherin-), MDA-MB-231 (E-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 20874-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 20874-1-AP
IP result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.
IHC staining of mouse colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 20874-1-AP
FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.
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FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.
IF Staining of mouse colon using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse colon using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human breast cancer using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse pancreas using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human breast cancer using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human breast cancer using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 20874-1-AP
Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).
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Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).
IF Staining of mouse colon using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of mouse colon using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of mouse breast cancer using 20874-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using E-cadherin antibody (20874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 20874-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 20874-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

E-cadherin Polyclonal antibody

The E-cadherin polyclonal antibody (20874-1-AP) is one of the most highly cited E-cadherin antibodies in the market. It is suitable for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA and has been referenced in over 2,454 publications.

Cat No. 20874-1-AP

Publications(2454)

Reviews (11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA

Synonyms

Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (2454)
Protocols
Reviews (11)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected in	A431 cells
Positive IHC detected in	mouse colon tissue, human breast cancer tissue, human colon tissue, human prostate cancer tissue, mouse skin tissue, rat colon tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue, human breast cancer tissue, mouse small intestine tissue, mouse pancreas tissue
Positive IF-Fro detected in	mouse colon tissue, mouse breast cancer
Positive IF/ICC detected in	MCF-7 cells, A431 cells

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:200000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 2135 publications below
IHC	See 400 publications below
IF	See 455 publications below
IP	See 1 publications below
CoIP	See 7 publications below

Product Information

20874-1-AP targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14973 Product name: Recombinant human E-cadherin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 373-622 aa of BC141838 Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI Predict reactive species
Full Name	cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight	882 aa, 97 kDa
Observed Molecular Weight	120-125 kDa
GenBank Accession Number	BC141838
Gene Symbol	E-cadherin
Gene ID (NCBI)	999
RRID	AB_10697811
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12830
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Protocols

Product Specific Protocols
IF protocol for E-cadherin antibody 20874-1-AP	Download protocol
IHC protocol for E-cadherin antibody 20874-1-AP	Download protocol
IP protocol for E-cadherin antibody 20874-1-AP	Download protocol
WB protocol for E-cadherin antibody 20874-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Science Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice. Authors - Yanpu Chen View Article
mouse,human	WB,IHC	Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 Authors - Jin Wang View Article
human	IF	Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article
human,mouse	WB,IF	Adv Mater Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1 Authors - Shaoting Zheng View Article
human	IF	Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer Authors - Yifei Cheng View Article
rat,mouse,human	WB	Sci Transl Med MG53-mediated cell membrane repair protects against acute kidney injury. Authors - Pu Duann View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Dhanwini (Verified Customer) (09-24-2025)	GOOD Applications: Western Blot
FH Robert (Verified Customer) (09-24-2025)	Works as intended! Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HCEC
FH Sneha (Verified Customer) (09-24-2025)	GOOD Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 Cell Tissue Type: ARPE-19
FH Zeeshan (Verified Customer) (09-18-2025)	Work great Applications: Western Blot, Immunohistochemistry Primary Antibody Dilution: 1:5000 Cell Tissue Type: Muller glia
FH Michael (Verified Customer) (09-18-2025)	Works perfectly for western blots Applications: Western Blot, Immunofluorescence, Cell culture Primary Antibody Dilution: 1:5000 Cell Tissue Type: Corneal cells
FH Henry (Verified Customer) (09-12-2025)	Excellent product Perfect bands Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry Primary Antibody Dilution: 1:2000 Cell Tissue Type: Muller glia
FH Jimmy (Verified Customer) (05-20-2025)	Great results for western blot application Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: human lung cancer cells
FH Davide (Verified Customer) (11-22-2022)	The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking) Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Cholangiocyte organoids E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)
FH Sophie (Verified Customer) (02-07-2022)	Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues Applications: Immunofluorescence Primary Antibody Dilution: 1/1000
FH Kishor (Verified Customer) (01-30-2019)	This antibody working very good for human cancer cells in western blotting (1:1000) Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: H69, HUCCT-1
FH Martin (Verified Customer) (12-13-2017)	Applications: Western Blot, Immunofluorescence