• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for WB, IP, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF-P, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 20874-1-AP

Synonyms

CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of A431 using 20874-1-AP

    WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of DU 145 using 20874-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    MCF-7 (E-cadherin+), A431 (E-cadherin+), HeLa (E-cadherin-), MDA-MB-231 (E-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of mouse testis using 20874-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IP experiment of A431 using 20874-1-AP

    IP result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse skin using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse skin using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human prostate cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human prostate cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human breast cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human breast cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of MCF-7 using 20874-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of A431 using 20874-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of A431 using 20874-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of A431 using 20874-1-AP

    Immunofluorescent analysis of A431 cells using 20874-1-AP (E-cadherin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse embryo using 20874-1-AP

    E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). Image credit: @Immunofluorescence on Instagram

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse small intestine using 20874-1-AP

    FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.

Tested Applications

Positive WB detected inA431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, A431 cells, human breast cancer tissue, MCF-7 cells, mouse embryo tissue, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20874-1-AP targets E-cadherin in WB, IP, IHC, IF-P, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
RRIDAB_10697811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

Authors - Yanpu Chen
View Article
human,mouseWB,IHC

Mol Cancer

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

Authors - Jin Wang
View Article
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
View Article
human, rat, mouseWB

Sci Transl Med

MG53-mediated cell membrane repair protects against acute kidney injury.

Authors - Pu Duann
View Article
mouseWB

ACS Nano

Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.

Authors - Han Lin
View Article
humanWB

Nat Commun

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis

Authors - Jeonghyun Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Davide (Verified Customer) (11-22-2022)

The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)
FH

Sophie (Verified Customer) (02-07-2022)

Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Martin (Verified Customer) (12-13-2017)

  • Applications: Western Blot, Immunofluorescence