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  • Featured Product
  • KD/KO Validated

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 20874-1-AP

Synonyms

CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of A431 using 20874-1-AP

    WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of DU 145 using 20874-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis using 20874-1-AP

    MCF-7 (E-cadherin+), A431 (E-cadherin+), HeLa (E-cadherin-), MDA-MB-231 (E-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    WB analysis of mouse testis using 20874-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IP experiment of A431 using 20874-1-AP

    IP result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of rat stomach using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse skin using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of mouse skin using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human prostate cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human prostate cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of rat colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of rat colon using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of rat stomach using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human breast cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IHC staining of human breast cancer using 20874-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse small intestine using 20874-1-AP

    FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of human breast cancer using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of mouse colon using 20874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of A431 using 20874-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using E-cadherin Polyclonal antibody (20874-1-AP)

    IF Staining of MCF-7 using 20874-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human colon tissue, human prostate cancer tissue, mouse skin tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, human breast cancer tissue, mouse small intestine tissue
Positive IF-Fro detected inmouse colon tissue
Positive IF/ICC detected inMCF-7 cells, A431 cells

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:200000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20874-1-AP targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
RRIDAB_10697811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

Authors - Yanpu Chen
View Article
human,mouseWB,IHC

Mol Cancer

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

Authors - Jin Wang
View Article
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
View Article
human, rat, mouseWB

Sci Transl Med

MG53-mediated cell membrane repair protects against acute kidney injury.

Authors - Pu Duann
View Article
mouseWB

ACS Nano

Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.

Authors - Han Lin
View Article
humanWB

Nat Commun

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis

Authors - Jeonghyun Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Davide (Verified Customer) (11-22-2022)

The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)
FH

Sophie (Verified Customer) (02-07-2022)

Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Martin (Verified Customer) (12-13-2017)

  • Applications: Western Blot, Immunofluorescence