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Tested Applications
| Positive IHC detected in | human colon tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 2 publications below |
Product Information
18841-1-AP targets DNAH2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | dynein, axonemal, heavy chain 2 |
| Calculated Molecular Weight | 508 kDa |
| GenBank Accession Number | NM_020877 |
| Gene Symbol | DNAH2 |
| Gene ID (NCBI) | 146754 |
| RRID | AB_2878565 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P225 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAH2, also named as DNAHC2, DNHD3 and KIAA1503, belongs to the dynein heavy chain family. DNAH2 force generating protein of respiratory cilia and produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. DNAH2 involved in sperm motility; implicated in sperm flagellar assembly.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNAH2 antibody 18841-1-AP | Download protocol |
| IF protocol for DNAH2 antibody 18841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Andrology A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans | ||
Asian J Androl A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility |
