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  • Immunoprecipitation (IP) experiment of HEK-293 cells using DMXL1 Polyclonal antibody (24413-1-AP)

    IP experiment of HEK-293 using 24413-1-AP

    IP result of anti-DMXL1 (IP:24413-1-AP, 4ug; Detection:24413-1-AP 1:2500) with HEK-293 cells lysate 960 ug.

DMXL1 Polyclonal antibody

DMXL1 Polyclonal Antibody for IP, ELISA
Cat No. 24413-1-AP
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

Dmx like 1, DmX like protein 1, DMXL1, X like 1 protein, XL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24413-1-AP targets DMXL1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMXL1 fusion protein Ag19846 Predict reactive species
Full Name Dmx-like 1
Calculated Molecular Weight 3027 aa, 338 kDa
Observed Molecular Weight338 kDa
GenBank Accession NumberBC150616
Gene Symbol DMXL1
Gene ID (NCBI) 1657
RRIDAB_2879532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y485
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMXL1 is a member of the WD repeat superfamily of proteins, which have regulatory functions. DMXL1 is expressed in many tissue types including several types of eye tissue, and it has been associated with ocular phenotypes. In addition, it is upregulated in cultured cells that overexpress growth factor independence 1B, a transcription factor that is essential for hematopoietic cell development.

Protocols

Product Specific Protocols
IP protocol for DMXL1 antibody 24413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Function (Oxf)

Dmxl1 is an Essential Mammalian Gene that is Required for V-ATPase Assembly and Function In Vivo

Authors - Amity F Eaton
View Article