HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26385-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 26385-1-AP
IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 26385-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.
HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26385-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse liver using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 26385-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 26385-1-AP
IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.
IF/ICC Figures
IF Staining of HepG2 using 26385-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DDX5 Polyclonal antibody
Catalog Number
26385-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, rat, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
