- Featured Product
- KD/KO Validated
CYP51A1 Polyclonal antibody
CYP51A1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 13431-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, rat liver tissue, HuH-7 cells |
| Positive IP detected in | mouse testis tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 26 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
13431-1-AP targets CYP51A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP51A1 fusion protein Ag4235 |
| Full Name | cytochrome P450, family 51, subfamily A, polypeptide 1 |
| Calculated Molecular Weight | 509 aa, 57 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC032322 |
| Gene Symbol | CYP51A1 |
| Gene ID (NCBI) | 1595 |
| RRID | AB_2088571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| IF protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| IP protocol for CYP51A1 antibody 13431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (12-01-2020) | Clear band with clean background
 |
