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CYP2A6 Polyclonal antibody

CYP2A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21721-1-AP

Synonyms

Coumarin 7 hydroxylase, CYP2A, CYP2A3, CYP2A6, CYPIIA6, Cytochrome P450 2A6, Cytochrome P450 IIA3, Cytochrome P450(I), P450C2A, P450PB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP2A6 Polyclonal antibody (21721-1-AP)

    WB analysis using 21721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CYP2A6 Polyclonal antibody (21721-1-AP)

    WB analysis of mouse liver using 21721-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CYP2A6 Polyclonal antibody (21721-1-AP)

    WB analysis of HepG2 using 21721-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21721-1-AP (CYP2A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2A6 Polyclonal antibody (21721-1-AP)

    IHC staining of human liver using 21721-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CYP2A6 Polyclonal antibody (21721-1-AP)

    IHC staining of human liver using 21721-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2A6 Polyclonal antibody (21721-1-AP)

    IHC staining of human liver cancer using 21721-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2A6 Polyclonal antibody (21721-1-AP)

    IHC staining of human liver cancer using 21721-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21721-1-AP (CYP2A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, mouse lung tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 21721-1-AP is CYP2A6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP2A6 fusion protein Ag16377
Full Name cytochrome P450, family 2, subfamily A, polypeptide 6
Calculated Molecular Weight 494 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC096253
Gene Symbol CYP2A6
Gene ID (NCBI) 1548
RRIDAB_10791886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP2A6 antibody 21721-1-APDownload protocol
IHC protocol for CYP2A6 antibody 21721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells.

Authors - Ashley A Untereiner
View Article
human,mouseWB

Xenobiotica

Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.

Authors - Shotaro Uehara
View Article
humanWB

Sci Rep

Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells

Authors - Sunitha Kodidela
View Article
mouseWB

Front Toxicol

E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis

Authors - Bayan Alakhtar
View Article
mouse

Biochem Biophys Res Commun

Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer

Authors - Yuya Yoshida
View Article

Biochem Biophys Res Commun

Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer

Authors - Yuya Yoshida
View Article