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Cytochrome c Polyclonal antibody

Name: Cytochrome c Polyclonal antibody
Brand: Proteintech
SKU: 10993-1-AP
Rating: 5.00 (5 reviews)

Cytochrome c Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

489

Reviews (5)

Cat no : 10993-1-AP

Synonyms

CYC, CYCS, cyto c, Cytochrome c, cytochrome c, somatic, HCS, THC4

Validation Data Gallery

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WB analysis using 10993-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10993-1-AP
WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.
WB analysis using 10993-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10993-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10993-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 10993-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10993-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse eye using 10993-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10993-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10993-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, Jurkat cells, mouse kidney tissue, rat kidney tissue, C2C12 cells
Positive IHC detected in	human liver tissue, human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse eye tissue
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 459 publications below
IHC	See 25 publications below
IF	See 22 publications below
FC	See 1 publications below

Product Information

10993-1-AP targets Cytochrome c in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, Hippospongia, goat, chicken, sheep, monkey, hamster, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytochrome c fusion protein Ag1455
Full Name	cytochrome c, somatic
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12-15 kDa
GenBank Accession Number	BC009578
Gene Symbol	CYCS
Gene ID (NCBI)	54205
RRID	AB_2090467
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 10993-1-AP	Download protocol
IHC protocol for Cytochrome c antibody 10993-1-AP	Download protocol
IF protocol for Cytochrome c antibody 10993-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Authors - Tara E Tracy View Article
human	WB	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
human	WB	Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. Authors - Qichao Huang View Article
mouse	WB	EMBO J Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. Authors - Rongpan Bai View Article
human	IF	ACS Appl Mater Interfaces Mitochondrial Voltage-Dependent Anion Channel 1-Hexokinase-II Complex-Targeted Strategy for Melanoma Inhibition Using Designed Multiblock Peptide Amphiphiles. Authors - Fan Zhang View Article
chicken	WB	Sci Total Environ MitomiR-1736-3p regulates copper-induced mitochondrial pathway apoptosis by inhibiting AATF in chicken hepatocytes Authors - Yuanxu Li View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Kazu (Verified Customer) (12-14-2022)	Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse optic nerve
FH Azita (Verified Customer) (06-02-2021)	Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: NSC34
FH Ying (Verified Customer) (04-22-2021)	good antibody. work well with mouse cells Applications: Western Blot Primary Antibody Dilution: 1:4000 Cytochrome c Antibody Western Blot validation (1:4000 dilution) in (Cat no:10993-1-AP)
FH Chi (Verified Customer) (09-26-2019)	The antibody works very well with good signal and low background. Applications: Western Blot, Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse embryonic fibroblasts Cytochrome c Antibody Western Blot, validation (1:2000 dilution) in mouse embryonic fibroblasts (Cat no:10993-1-AP)
FH Xiaoping (Verified Customer) (10-22-2018)	The signal is good and the size is between 10-15kDa. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse fibroblast Cytochrome c Antibody Western Blot, validation (1:1000 dilution) in mouse fibroblast (Cat no:10993-1-AP)

Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Validation Data Gallery

WB analysis using 10993-1-AP

WB analysis of HEK-293 using 10993-1-AP

WB analysis using 10993-1-AP

WB analysis of rat liver using 10993-1-AP

WB analysis of mouse skeletal muscle using 10993-1-AP

WB analysis of rat skeletal muscle using 10993-1-AP

WB analysis of NIH/3T3 using 10993-1-AP

IHC staining of human liver using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

IHC staining of human liver using 10993-1-AP

IHC staining of human breast cancer using 10993-1-AP

IHC staining of human breast cancer using 10993-1-AP

IF Staining of mouse eye using 10993-1-AP

FC experiment of HepG2 using 10993-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Mitochondrial Voltage-Dependent Anion Channel 1-Hexokinase-II Complex-Targeted Strategy for Melanoma Inhibition Using Designed Multiblock Peptide Amphiphiles.

MitomiR-1736-3p regulates copper-induced mitochondrial pathway apoptosis by inhibiting AATF in chicken hepatocytes

Reviews

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