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Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

548

Reviews (5)

Cat no : 10993-1-AP

Synonyms

CYCS, CYC

Validation Data Gallery

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AllWBIHCIF-PFC (Intra)

WB analysis using 10993-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10993-1-AP
WB result of Cytochrome c antibody (10993-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
WB analysis of HEK-293 using 10993-1-AP
WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.
WB analysis using 10993-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10993-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10993-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 10993-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10993-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10993-1-AP (CYCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10993-1-AP (CYCS antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse eye using 10993-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 10993-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HepG2 using 10993-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10993-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, HEK-293 cells, HepG2 cells, C2C12 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected in	human liver tissue, human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue
Positive FC (Intra) detected in	HepG2 cells

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 514 publications below
IHC	See 29 publications below
IF	See 27 publications below
FC	See 1 publications below

Product Information

10993-1-AP targets Cytochrome c in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, hamster, sheep, goat, hippospongia
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytochrome c fusion protein Ag1455
Full Name	cytochrome c, somatic
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12-15 kDa
GenBank Accession Number	BC009578
Gene Symbol	Cytochrome c
Gene ID (NCBI)	54205
RRID	AB_2090467
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 10993-1-AP	Download protocol
IHC protocol for Cytochrome c antibody 10993-1-AP	Download protocol
IF protocol for Cytochrome c antibody 10993-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Authors - Tara E Tracy View Article
human	WB,IF	Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration Authors - Jianxiong Chen View Article
human	WB	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
mouse	WB	Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis Authors - Yujun Zhou View Article
human	WB	Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. Authors - Qichao Huang View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Kazu (Verified Customer) (12-14-2022)	Worked well for 4% PFA fixed mouse optic nerve using this antibody at dilution of 1:200. Little background observed. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse optic nerve
FH Azita (Verified Customer) (06-02-2021)	Western blot analysis using Cytochrome c polyclonal antibody in NSC34 cell line at dilution of 1:1000. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: NSC34
FH Ying (Verified Customer) (04-22-2021)	good antibody. work well with mouse cells Applications: Western Blot Primary Antibody Dilution: 1:4000 Cytochrome c Antibody Western Blot validation (1:4000 dilution) in (Cat no:10993-1-AP)
FH Chi (Verified Customer) (09-26-2019)	The antibody works very well with good signal and low background. Applications: Western Blot, Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse embryonic fibroblasts Cytochrome c Antibody Western Blot, validation (1:2000 dilution) in mouse embryonic fibroblasts (Cat no:10993-1-AP)
FH Xiaoping (Verified Customer) (10-22-2018)	The signal is good and the size is between 10-15kDa. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse fibroblast Cytochrome c Antibody Western Blot, validation (1:1000 dilution) in mouse fibroblast (Cat no:10993-1-AP)

Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10993-1-AP

WB analysis of HepG2 using 10993-1-AP

WB analysis of HEK-293 using 10993-1-AP

WB analysis using 10993-1-AP

WB analysis of rat liver using 10993-1-AP

WB analysis of mouse skeletal muscle using 10993-1-AP

WB analysis of rat skeletal muscle using 10993-1-AP

WB analysis of NIH/3T3 using 10993-1-AP

IHC staining of human liver using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

IHC staining of human liver using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

IHC staining of human breast cancer using 10993-1-AP

IHC staining of human breast cancer using 10993-1-AP

IF Staining of mouse eye using 10993-1-AP

IF Staining of mouse eye using 10993-1-AP

FC experiment of HepG2 using 10993-1-AP

Tested Applications

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Reviews

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