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CXCR7-Specific Polyclonal antibody

CXCR7-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14840-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of K-562 using 14840-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of mouse brain using 14840-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    WB analysis of C6 using 14840-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IP experiment of K-562 using 14840-1-AP

    IP result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CXCR7-Specific Polyclonal antibody (14840-1-AP)

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, C6 cells, mouse brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14840-1-AP targets CXCR7-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name chemokine (C-X-C motif) receptor 7
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_020311
Gene Symbol CXCR7
Gene ID (NCBI) 57007
RRIDAB_10646437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.

Protocols

Product Specific Protocols
WB protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IHC protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IP protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols