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LIX/CXCL5 Polyclonal antibody

LIX/CXCL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10809-1-AP

Synonyms

CXCL5, ENA-78(1-78), CXCL-5, CXCL 5, C-X-C motif chemokine 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIX/CXCL5 Polyclonal antibody (10809-1-AP)

    WB analysis using 10809-1-AP

    Untreated, PMA treated A549 cells were subjected to SDS PAGE followed by western blot with 10809-1-AP (CXCL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CXCL5 Polyclonal antibody (10809-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10809-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10809-1-AP (CXCL5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPMA treated A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10809-1-AP targets LIX/CXCL5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIX/CXCL5 fusion protein Ag1079
Full Name chemokine (C-X-C motif) ligand 5
Calculated Molecular Weight 12 kDa
Observed Molecular Weight8 kDa
GenBank Accession NumberBC008376
Gene Symbol CXCL5
Gene ID (NCBI) 6374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.

Protocols

Product Specific Protocols
WB protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
IHC protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Stem Cells

Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs: Insights from Single-Cell RNA Sequencing

Authors - Peng Huang
View Article