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WB analysis of HEK-293 using 13974-1-AP
WB result of CTNNA3 antibody (13974-1-AP, 1:500) with si-Control and si-CTNNA3 transfected HEK293 cells.
WB analysis of mouse testis using 13974-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13974-1-AP (CTNNA3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13974-1-AP
IP result of anti-CTNNA3 (IP:13974-1-AP, 5ug; Detection:13974-1-AP 1:1500) with mouse testis tissue lysate 4000ug.
IHC staining of human liver cancer using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse heart using 13974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13974-1-AP
Immunofluorescent analysis of HepG2 cells, using CTNNA3 antibody 13974-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

CTNNA3 Polyclonal antibody

Name: CTNNA3 Polyclonal antibody
Brand: Proteintech
SKU: 13974-1-AP

CTNNA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13974-1-AP

Publications(12)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

VR22, Catenin alpha-3, Catenin alpha 3, Cadherin-associated protein, Alpha T-catenin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse testis tissue, HEK-293
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human liver cancer tissue, human heart tissue, human placenta tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

13974-1-AP targets CTNNA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CTNNA3 fusion protein Ag5008 Predict reactive species
Full Name	catenin (cadherin-associated protein), alpha 3
Calculated Molecular Weight	100 kDa
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC030646
Gene Symbol	CTNNA3
Gene ID (NCBI)	29119
RRID	AB_2088078
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UI47
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CTNNA3 antibody 13974-1-AP	Download protocol
IHC protocol for CTNNA3 antibody 13974-1-AP	Download protocol
IF protocol for CTNNA3 antibody 13974-1-AP	Download protocol
IP protocol for CTNNA3 antibody 13974-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Global landscape of HIV-human protein complexes. Authors - Stefanie Jäger View Article
mouse	IF	Cell Stem Cell An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling. Authors - Allison P Kann View Article
mouse	IF	Circ Res Alpha-Catenins Control Cardiomyocyte Proliferation by Regulating Yap Activity. Authors - Jifen Li View Article
human	WB	Nat Commun Cell-cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas. Authors - Fanjul-Fernández Miriam M KD Validated View Article
mouse	IF	Elife A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation. Authors - Jie Wang View Article
mouse	IF,WB	Development α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart. Authors - Alexia Vite KO Validated View Article