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Casein Kinase 1 Epsilon Polyclonal antibody

Casein Kinase 1 Epsilon Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11230-1-AP

Synonyms

Casein Kinase 1 epsilon, casein kinase 1, epsilon, CKI epsilon, CKIe, CSNK1E, HCKIE

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    WB analysis of A431 using 11230-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    WB analysis of mouse brain using 11230-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human breast cancer using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human breast cancer using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human heart using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human heart using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human kidney using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human kidney using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human testis using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human testis using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human ovary using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human ovary using 11230-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11230-1-AP targets Casein Kinase 1 Epsilon in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Casein Kinase 1 Epsilon fusion protein Ag1742
Full Name casein kinase 1, epsilon
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC006490
Gene Symbol CK1 epsilon
Gene ID (NCBI) 1454
RRIDAB_2084501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332).

Protocols

Product Specific Protocols
WB protocol for Casein Kinase 1 Epsilon antibody 11230-1-APDownload protocol
IHC protocol for Casein Kinase 1 Epsilon antibody 11230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
View Article