Various lysates were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17720-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 17720-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HL-60 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17720-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 17720-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
rat lung tissue were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 17720-1-AP
IP result of anti-CSK (IP:17720-1-AP, 4ug; Detection:17720-1-AP 1:800) with HL-60 cells lysate 1000ug.
Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A431 using 17720-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CSK antibody (17720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CSK antibody (17720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung tissue, human skin tissue, human placenta tissue, human kidney tissue, human brain tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).
Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.
Various lysates were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17720-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 17720-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17720-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 17720-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human placenta using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human brain using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17720-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HL-60 using 17720-1-AP
IP result of anti-CSK (IP:17720-1-AP, 4ug; Detection:17720-1-AP 1:800) with HL-60 cells lysate 1000ug.
IF/ICC Figures
IF Staining of A431 using 17720-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CSK antibody (17720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CSK Polyclonal antibody
Catalog Number
17720-1-AP
Citations
5
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, rat, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
