HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 17739-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17739-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17739-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17739-1-AP
IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:1000) with HeLa cells lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 17739-1-AP
Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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HeLa cells, human brain tissue, mouse brain tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 17739-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17739-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17739-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 17739-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17739-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HeLa using 17739-1-AP
IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:1000) with HeLa cells lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 17739-1-AP
Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CPSF2 Polyclonal antibody
Catalog Number
17739-1-AP
Citations
2
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
