• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CPLX4 Polyclonal antibody

CPLX4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21222-1-AP

Synonyms

CPXIV, CPX IV, Complexin-4, Complexin IV, complexin 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.

  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    IP experiment of Y79 using 21222-1-AP

    IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of mouse eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat eye tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of rat eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of human brain using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inmouse eye tissue, human brain tissue, rat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21222-1-AP targets CPLX4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX4 fusion protein Ag15661
Full Name complexin 4
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC106887
Gene Symbol CPLX4
Gene ID (NCBI) 339302
RRIDAB_10733348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CPLX4 antibody 21222-1-APDownload protocol
IHC protocol for CPLX4 antibody 21222-1-APDownload protocol
IP protocol for CPLX4 antibody 21222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols