Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 21222-1-AP
WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.
WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.
WB analysis of Y79 using 21222-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Y79 using 21222-1-AP
IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat eye using 21222-1-AP
Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 21222-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of rat eye using 21222-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using CPLX4 antibody (21222-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using CPLX4 antibody (21222-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse eye tissue, human brain tissue, rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
rat eye tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
21222-1-AP targets CPLX4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 21222-1-AP
WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.
WB analysis of Y79 using 21222-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse eye using 21222-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat eye using 21222-1-AP
Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 21222-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of Y79 using 21222-1-AP
IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.
IF-P Figures
IF Staining of rat eye using 21222-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using CPLX4 antibody (21222-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CPLX4 Polyclonal antibody
Catalog Number
21222-1-AP
Citations
-
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
