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  • Featured Product
  • KD/KO Validated

COXIV Polyclonal antibody

COXIV Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11242-1-AP

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1

Filter:
  • Western Blot (WB) analysis of various lysates using COXIV Polyclonal antibody (11242-1-AP)

    WB analysis using 11242-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using COXIV Polyclonal antibody (11242-1-AP)

    WB analysis of HepG2 using 11242-1-AP

    WB result of COXIV antibody (11242-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using COXIV Polyclonal antibody (11242-1-AP)

    WB analysis using 11242-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using COXIV Polyclonal antibody (11242-1-AP)

    WB analysis of rat brain using 11242-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using COXIV Polyclonal antibody (11242-1-AP)

    WB analysis of rat liver using 11242-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using COXIV Polyclonal antibody (11242-1-AP)

    IP experiment of mouse skeletal muscle using 11242-1-AP

    IP result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human prostate cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human prostate cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human pancreas cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human pancreas cancer using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human heart using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11242-1-AP (COXIV antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Polyclonal antibody (11242-1-AP)

    IHC staining of human heart using 11242-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11242-1-AP (COXIV antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COXIV Polyclonal antibody (11242-1-AP)

    IF Staining of HepG2 using 11242-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (11242-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

"COXIV Antibodies" Comparison

View side-by-side comparison of COXIV antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, rat liver tissue, rat brain tissue, MCF-7 cells, NIH/3T3 cells, Jurkat cells, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11242-1-AP targets COXIV in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COXIV fusion protein Ag1640
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated Molecular Weight19.6 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC021236
Gene Symbol COX IV
Gene ID (NCBI) 1327
RRIDAB_2085278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

Protocols

Product Specific Protocols
WB protocol for COXIV antibody 11242-1-APDownload protocol
IHC protocol for COXIV antibody 11242-1-APDownload protocol
IF protocol for COXIV antibody 11242-1-APDownload protocol
IP protocol for COXIV antibody 11242-1-APDownload protocol
FC protocol for COXIV antibody 11242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
View Article
human,mouseWB,IF

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
View Article
mouseWB

Nat Metab

Time of exercise differentially impacts bone growth in mice

Authors - Shaoling Yu
View Article
humanWB

Cell Metab

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Authors - Kiran Kurmi
View Article
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
View Article
mouseWB

Cell Res

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Authors - Tingting Hou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Jimmy (Verified Customer) (02-27-2024)

Strong signal in primary human cell fractions.

  • Applications: Western Blot
FH

Maria (Verified Customer) (08-17-2021)

Good ab, works well 1:1000 in BSA 3% O/N 4ºC incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human primary fibroblasts
COXIV Antibody Western Blot validation (1:1000 dilution) in human primary fibroblasts (Cat no:11242-1-AP)
FH

Mohammed (Verified Customer) (08-19-2020)

Very strong staining in the midpiece of the sperm tail.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: (1:200)
  • Cell Tissue Type: Isolated sperm
FH

SITING (Verified Customer) (07-13-2020)

THIS IS A GOOD ANTIBODY, CAN SEE CLEAR BAND

  • Applications: Western Blot,
COXIV Antibody Western Blot, validation ( dilution) in (Cat no:11242-1-AP)