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KD/KO Validated

COL6A1 Polyclonal antibody

COL6A1 Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

Cat No. 17023-1-AP

Publications(56)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IF-P, IP, ELISA

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Proteintech Guarantee

Filter: AllWBIPIHCIF-PIF

WB analysis using 17023-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293T using 17023-1-AP
WB result of Collagen Type VI antibody (17023-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type VI transfected HEK-293T cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human skeletal muscle using 17023-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human heart using 17023-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse heart using 17023-1-AP
IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human colon using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human colon using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse skeletal muscle using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse skeletal muscle tissue using 17023-1-AP
IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

Overview

Tested Applications
Product Information
Publications (56)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, HEK-293T cells, human heart tissue, human skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human skin cancer tissue, human spleen tissue, human testis tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human colon tissue, mouse skeletal muscle tissue
Positive IF detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17023-1-AP targets COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, zebrafish, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10288 Product name: Recombinant human COL6A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 680-1028 aa of BC052575 Sequence: SIRNVQELKEAIKSLQWMAGGTFTGEALQYTRDQLLPPSPNNRIALVITDGRSDTQRDTTPLNVLCSPGIQVVSVGIKDVFDFIPGSDQLNVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG Predict reactive species
Full Name	collagen, type VI, alpha 1
Calculated Molecular Weight	1028 aa, 109 kDa
Observed Molecular Weight	140 kDa
GenBank Accession Number	BC052575
Gene Symbol	COL6A1
Gene ID (NCBI)	1291
RRID	AB_2229737
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12109
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
IF protocol for COL6A1 antibody 17023-1-AP	Download protocol
IHC protocol for COL6A1 antibody 17023-1-AP	Download protocol
IP protocol for COL6A1 antibody 17023-1-AP	Download protocol
WB protocol for COL6A1 antibody 17023-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
zebrafish	WB,IF	Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis Authors - Ying Liu View Article
human	WB,IHC,IF	Drug Resist Updat Endothelial cells sense temozolomide resistance to facilitate monocyte-derived macrophage infiltration in glioblastoma. Authors - Wei Gao KD Validated View Article
human, mouse, rat	IF	Nat Commun Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling. Authors - Yuxiang Hu View Article
mouse	IF	Nat Commun Metabolic adaptation drives self-organization during skin organoid morphogenesis. Authors - Jingwei Jiang View Article
mouse	IF	Nat Commun Heterogeneous fibroblasts contribute to fibrotic scar formation after spinal cord injury in mice and monkeys Authors - Xiaoyu Xue View Article
rat	IF	ACS Nano Vitamin A-Integrated Cinnamaldehyde Nanoemulsion: A Nanotherapeutic Approach To Counteract Liver Fibrosis via Gut-Liver Axis Modulation Authors - Xia Niu View Article