COG3 Polyclonal antibody

COG3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11130-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

COG complex subunit 3, COG3, p94, SEC34

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse liver, rat liver
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11130-1-AP targets COG3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG3 fusion protein Ag1610 Predict reactive species
Full Name component of oligomeric golgi complex 3
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC038953
Gene Symbol COG3
Gene ID (NCBI) 83548
RRIDAB_2081402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JB2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COG3 antibody 11130-1-APDownload protocol
IHC protocol for COG3 antibody 11130-1-APDownload protocol
IP protocol for COG3 antibody 11130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cancer Cell

Systematic Functional Annotation of Somatic Mutations in Cancer.

Authors - Patrick Kwok-Shing Ng
humanIP

Cancer Cell

A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.

Authors - Xinxin Peng
humanWB

Cancer Cell

The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers.

Authors - Leng Han
humanWB,IP

Cancer Immunol Res

PMID: 32265228

Authors - Jiyong Liang

Cancer Res

Sustained Aurora Kinase B Expression Confers Resistance to PI3K Inhibition in Head and Neck Squamous Cell Carcinoma

Authors - Pooja A Shah
humanWB

Sci Rep

Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer

Authors - Andrea Martisova
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