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  • Western Blot (WB) analysis of mouse brain tissue using CLEC12B Polyclonal antibody (26077-1-AP)

    WB analysis of mouse brain using 26077-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CLEC12B Polyclonal antibody (26077-1-AP)

    WB analysis of rat brain using 26077-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CLEC12B Polyclonal antibody (26077-1-AP)

    WB analysis of K-562 using 26077-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CLEC12B Polyclonal antibody (26077-1-AP)

    WB analysis of mouse spleen using 26077-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26077-1-AP (CLEC12B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CLEC12B Polyclonal antibody

CLEC12B Polyclonal Antibody for WB, ELISA
Cat No. 26077-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CLEC12B, Macrophage antigen H, UNQ5782

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse brain tissue, K-562 cells, mouse spleen tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26077-1-AP targets CLEC12B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC12B fusion protein Ag23396 Predict reactive species
Full Name C-type lectin domain family 12, member B
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC136805
Gene Symbol CLEC12B
Gene ID (NCBI) 387837
RRIDAB_2880365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2HXU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC12B (C-type lectin domain family 12 member B) is a single-pass type II membrane protein of the C-type lectin-like family. CLEC12B acts as an inhibitory receptor on myeloid cells. CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. (PMID: 17562706)

Protocols

Product Specific Protocols
WB protocol for CLEC12B antibody 26077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols