CLASP2 Polyclonal antibody
CLASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 12942-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12942-1-AP targets CLASP2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CLASP2 fusion protein Ag4012 |
| Full Name | cytoplasmic linker associated protein 2 |
| Calculated Molecular Weight | 1294 aa, 141 kDa |
| Observed Molecular Weight | 140-160 kDa |
| GenBank Accession Number | BC029035 |
| Gene Symbol | CLASP2 |
| Gene ID (NCBI) | 23122 |
| RRID | AB_2260448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLASP2 antibody 12942-1-AP | Download protocol |
| IHC protocol for CLASP2 antibody 12942-1-AP | Download protocol |
| IF protocol for CLASP2 antibody 12942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Bone Miner Res Regulation of bone resorption and sealing zone formation in osteoclasts occurs through protein kinase B-mediated microtubule stabilization. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah (Verified Customer) (07-06-2020) | 30ug HEK protein loaded, and CLASP2 levels detected for control cells or cells transfected with CLASP2 siRNA
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