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  • Featured Product
  • KD/KO Validated

CDK4 Polyclonal antibody

CDK4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 11026-1-AP

Synonyms

PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK4 Polyclonal antibody (11026-1-AP)

    WB analysis using 11026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDK4 Polyclonal antibody (11026-1-AP)

    WB analysis of HeLa using 11026-1-AP

    WB result of CDK4 antibody (11026-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells.

  • Western Blot (WB) analysis of rat pancreas tissue using CDK4 Polyclonal antibody (11026-1-AP)

    WB analysis of rat pancreas using 11026-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CDK4 Polyclonal antibody (11026-1-AP)

    WB analysis using 11026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CDK4 Polyclonal antibody (11026-1-AP)

    IP experiment of HeLa using 11026-1-AP

    IP result of anti-CDK4 (IP:11026-1-AP, 3ug; Detection:11026-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human breast cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK4 Polyclonal antibody (11026-1-AP)

    IHC staining of human breast cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CDK4 Polyclonal antibody (11026-1-AP)

    IF Staining of MCF-7 using 11026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (11026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using CDK4 Polyclonal antibody (11026-1-AP)

    FC experiment of MCF-7 using 11026-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDK4 Polyclonal antibody (11026-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, rat pancreas tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11026-1-AP targets CDK4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK4 fusion protein Ag1505
Full Name cyclin-dependent kinase 4
Calculated Molecular Weight 34 kDa
Observed Molecular Weight30-34 kDa
GenBank Accession NumberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
RRIDAB_2078702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

Protocols

Product Specific Protocols
WB protocol for CDK4 antibody 11026-1-APDownload protocol
IHC protocol for CDK4 antibody 11026-1-APDownload protocol
IF protocol for CDK4 antibody 11026-1-APDownload protocol
IP protocol for CDK4 antibody 11026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
View Article
humanIHC,WB

Mol Cancer

The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC.

Authors - Xiaosong Wang
View Article
humanWB

Sci Adv

The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis

Authors - Zhenlong Yu
View Article
humanWB

Nat Commun

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Authors - Youzhou Sang
View Article
humanWB

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su
View Article
humanWB

J Pineal Res

Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.

Authors - Zhenlong Yu
View Article