CDC25C Monoclonal antibody

CDC25C Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, FC

Conjugate

Unconjugated

CloneNo.

2B6B12

Cat no : 66912-1-Ig

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66912-1-Ig targets CDC25C in WB, FC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC25C fusion protein Ag10240
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight 46-53 kDa
GenBank accession numberBC019089
Gene symbol CDC25C
Gene ID (NCBI) 995
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Protocols

Product Specific Protocols
WB protocol for CDC25C antibody 66912-1-IgDownload protocol
FC protocol for CDC25C antibody 66912-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oncol Lett

Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis.

Authors - Jun Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angélique (Verified Customer) (11-25-2021)

on 20ug total proteins

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: murine hepatocytes
CDC25C Antibody Western Blot validation (1/2000 dilution) in murine hepatocytes (Cat no:66912-1-Ig)