• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10252-1-AP

Synonyms

p55CDC, CDC20A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis using 10252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis of HeLa using 10252-1-AP

    WB result of Cdc20 antibody (10252-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cdc20 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis using 10252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis using 10252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis of HL-60 using 10252-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using Cdc20 Polyclonal antibody (10252-1-AP)

    WB analysis of PC-3 using 10252-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using Cdc20 Polyclonal antibody (10252-1-AP)

    IP experiment of Jurkat using 10252-1-AP

    IP result of anti-Cdc20 (IP:10252-1-AP, 4ug; Detection:10252-1-AP 1:15000) with Jurkat cells lysate 1400 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human breast cancer using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human breast cancer using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human colon cancer using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (Cdc20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human colon cancer using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human colon cancer using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human urothelial carcinoma using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cdc20 Polyclonal antibody (10252-1-AP)

    IHC staining of human urothelial carcinoma using 10252-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cdc20 Polyclonal antibody (10252-1-AP)

    IF Staining of HeLa using 10252-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cdc20 antibody (10252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells, HL-60 cells, Jurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10252-1-AP targets Cdc20 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cdc20 fusion protein Ag0295
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
RRIDAB_2229016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protocols

Product Specific Protocols
WB protocol for Cdc20 antibody 10252-1-APDownload protocol
IHC protocol for Cdc20 antibody 10252-1-APDownload protocol
IF protocol for Cdc20 antibody 10252-1-APDownload protocol
IP protocol for Cdc20 antibody 10252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
View Article
humanWB

Elife

Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.

Authors - James Holder
View Article
humanWB

Int J Mol Sci

Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.

Authors - Yang Gao
  • KD Validated
View Article
humanWB

Cell Death Dis

Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.

Authors - Yanlin Huang
View Article
humanWB

Aging (Albany NY)

The bioinformatics and experimental analysis of the novel roles of virus infection-associated gene CDC20 for prognosis and immune infiltration in hepatocellular carcinoma.

Authors - Juanni Li
View Article