Validation Data Gallery
|Positive WB detected in||A375 cells, HeLa cells, A549 cells, L02 cells, HepG2 cells|
|Positive IHC detected in||human ovary tumor tissue, human colon cancer tissue, human breast cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60232-1-Ig is CD9 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD9 fusion protein Ag14529|
|Full Name||CD9 molecule|
|Calculated molecular weight||228 aa, 25 kDa|
|Observed molecular weight||23-27 kDa|
|GenBank accession number||BC011988|
|Gene ID (NCBI)||928|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
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