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Tested Applications
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 5 publications below |
Product Information
17325-1-AP targets CD1a in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD1a fusion protein Ag11304 Predict reactive species |
| Full Name | CD1a molecule |
| Calculated Molecular Weight | 327 aa, 37 kDa |
| GenBank Accession Number | BC031645 |
| Gene Symbol | CD1A |
| Gene ID (NCBI) | 909 |
| RRID | AB_10638479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1a is a 50-kDa glycoprotein. It has a heterodimer form with molecular weight of 65-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD1a antibody 17325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Emerg Microbes Infect CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis | ||
Cancer Immunol Immunother Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas. | ||
Stem Cell Res Ther Human umbilical cord mesenchymal stem cell treatment alleviates symptoms in an atopic dermatitis-like mouse model | ||
BMC Med Genomics Establishment and validation of an immune infiltration predictive model for ovarian cancer | ||
Aging (Albany NY) Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma |
