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CD19 Polyclonal antibody

CD19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27949-1-AP

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12



Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, mouse colon tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27949-1-AP targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD19 fusion protein Ag27656
Full Name CD19 molecule
Calculated Molecular Weight 556 aa, 61 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
RRIDAB_2881019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 27949-1-APDownload protocol
IHC protocol for CD19 antibody 27949-1-APDownload protocol
IF protocol for CD19 antibody 27949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Adv Res

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

Authors - Haonan Ruan
humanIHC

Int J Mol Sci

CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing

Authors - Chen Su
humanWB

Int J Biol Macromol

Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 + DCs

Authors - Changwei Zhang
humanIF

bioRxiv

A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies

Authors - José Alejandro Bohórquez
humanIHC

Cancer Lett

Comprehensive molecular characterization of long-term glioblastoma survivors

Authors - Hao Xu
IHC

J Ethnopharmacol

Effective substances and molecular mechanisms guided by network pharmacology: An example study of Scrophulariae Radix treatment of hyperthyroidism and thyroid hormone-induced liver and kidney injuries

Authors - Meng-Yuan Sheng