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CaMKII beta-Specific Polyclonal antibody

CaMKII beta-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55218-1-AP

Synonyms

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta, CaMKII beta-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    WB analysis using 55218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55218-1-AP (CaMKII beta-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    IHC staining of human skeletal muscle using 55218-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    IHC staining of human skeletal muscle using 55218-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    IHC staining of human brain using 55218-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    IHC staining of human brain using 55218-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CaMKII beta-Specific Polyclonal antibody (55218-1-AP)

    IF Staining of HEK-293 using 55218-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55218-1-AP (CaMKII beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, Hela cells, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman skeletal muscle tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55218-1-AP targets CaMKII beta-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium/calmodulin-dependent protein kinase II beta
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberNM_001220
Gene Symbol CaMKII beta
Gene ID (NCBI) 816
RRIDAB_11183766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII beta (Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAMK2B, CAM2 or CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals (PMID:22578382). CAMK2B has 8 isoforms produced by alternative splicing with the molecular weight 50-73 kDa. This antibody is specific to CAMK2B and can recognize all the isoforms except isofom 7.

Protocols

Product Specific Protocols
WB protocol for CaMKII beta-Specific antibody 55218-1-APDownload protocol
IHC protocol for CaMKII beta-Specific antibody 55218-1-APDownload protocol
IF protocol for CaMKII beta-Specific antibody 55218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols