Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13670-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 13670-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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HEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected in
human liver cancer tissue, human kidney tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13670-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 13670-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).
IF/ICC Figures
IF Staining of HeLa using 13670-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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