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KHDC3L Polyclonal antibody

KHDC3L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24989-1-AP

Synonyms

C6orf221, ECAT1, Filia, KHDC3L

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    WB analysis of human testis using 24989-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24989-1-AP (KHDC3L antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    IHC staining of human liver cancer using 24989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24989-1-AP (KHDC3L antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KHDC3L Polyclonal antibody (24989-1-AP)

    IHC staining of human liver cancer using 24989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24989-1-AP (KHDC3L antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KHDC3L Polyclonal antibody (24989-1-AP)

    IF Staining of HepG2 using 24989-1-AP

    Immunofluorescent analysis of HepG2 cells using 24989-1-AP (KHDC3L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24989-1-AP is KHDC3L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KHDC3L fusion protein Ag21790
Full Name chromosome 6 open reading frame 221
Calculated Molecular Weight 217 aa, 24 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberBC137160
Gene Symbol KHDC3L
Gene ID (NCBI) 154288
RRIDAB_2879833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KHDC3L (also known as Filia and ES cell-associated transcript1 [Ecat1]) as a key regulator in safeguarding the genomic stability of mouse early embryonic cells. KHDC3L is critical in preventing the replication associated DNA DSBs in epiblast cells (PMID: 29125140). Importantly, by analyzing KHDC3L mutations causes recurrent pregnancy loss by inducing genomic instability of human early embryonic cells (PMID: 31609975).

Protocols

Product Specific Protocols
WB protocol for KHDC3L antibody 24989-1-APDownload protocol
IHC protocol for KHDC3L antibody 24989-1-APDownload protocol
IF protocol for KHDC3L antibody 24989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols