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  • Western Blot (WB) analysis of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    WB analysis of HeLa using 24797-1-AP

    WB result of C19orf61 antibody (24797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf61 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    WB analysis of HeLa using 24797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24797-1-AP (C19orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using C19orf61 Polyclonal antibody (24797-1-AP)

    IP experiment of HeLa using 24797-1-AP

    IP result of anti-C19orf61 (IP:24797-1-AP, 3ug; Detection:24797-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C19orf61 Polyclonal antibody (24797-1-AP)

    IF Staining of U2OS using 24797-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C19orf61 antibody (24797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

C19orf61 Polyclonal antibody

C19orf61 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24797-1-AP
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

SMG9, Protein SMG9, Nonsense-mediated mRNA decay factor SMG9, F17127_1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24797-1-AP targets C19orf61 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf61 fusion protein Ag14965 Predict reactive species
Full Name chromosome 19 open reading frame 61
Calculated Molecular Weight 520 aa, 58 kDa
Observed Molecular Weight 57-65 kDa
GenBank Accession NumberBC008869
Gene Symbol C19orf61
Gene ID (NCBI) 56006
RRIDAB_2879731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0W8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C19orf61 antibody 24797-1-APDownload protocol
IF protocol for C19orf61 antibody 24797-1-APDownload protocol
IP protocol for C19orf61 antibody 24797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates

Authors - Yong Dai
  • KD Validated
View Article