• Featured Product
  • KD/KO Validated

BMI1 Monoclonal antibody

BMI1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, monkey







Cat no : 66161-1-Ig


BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

Tested Applications

Positive WB detected inPC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOS-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66161-1-Ig targets BMI1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BMI1 fusion protein Ag21284
Full Name BMI1 polycomb ring finger oncogene
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37-43 kDa
GenBank Accession NumberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.


Product Specific Protocols
WB protocol for BMI1 antibody 66161-1-IgDownload protocol
IHC protocol for BMI1 antibody 66161-1-IgDownload protocol
IF protocol for BMI1 antibody 66161-1-IgDownload protocol
IP protocol for BMI1 antibody 66161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai


Inhibition of BMI1 induces autophagy-mediated necroptosis.

Authors - Anindya Dey
  • KD Validated

Clin Transl Med

Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.

Authors - Haiyun Chen

J Bone Miner Res

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription

Authors - Qiuyi Wang

Aging (Albany NY)

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Authors - Zhengwei Zhang

Mol Cancer Ther

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Authors - Anindya Dey