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BLVRB Polyclonal antibody

BLVRB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17727-1-AP

Synonyms

BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLVRB Polyclonal antibody (17727-1-AP)

    WB analysis using 17727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using BLVRB Polyclonal antibody (17727-1-AP)

    WB analysis of HepG2 using 17727-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using BLVRB Polyclonal antibody (17727-1-AP)

    WB analysis of L02 using 17727-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    WB analysis of human liver using 17727-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    WB analysis of rat liver using 17727-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    IHC staining of human liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using BLVRB Polyclonal antibody (17727-1-AP)

    IHC staining of rat kidney using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    IHC staining of rat liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    IHC staining of human liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using BLVRB Polyclonal antibody (17727-1-AP)

    IHC staining of human liver using 17727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17727-1-AP targets BLVRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLVRB fusion protein Ag12124
Full Name biliverdin reductase B (flavin reductase (NADPH))
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC109371
Gene Symbol BLVRB
Gene ID (NCBI) 645
RRIDAB_2065087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BLVRB antibody 17727-1-APDownload protocol
IHC protocol for BLVRB antibody 17727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway.

Authors - Yinzhong Lu
View Article